Disinfection of medical bearings

Source: Bearing network time: 2017-10-12

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Method <br> Disinfection method â— WBY-1 microwave sterilizer shines for 1 minute â— Domestic 2% enhanced neutral glutaraldehyde soak for 20 minutes â— 0. 2% Xinjieer immersion for 20 minutes â— 75% alcohol cotton ball wipe test laboratory method The bearing was repeatedly scrubbed 5 times with a sterile cotton swab; the cotton swab was placed in 10 ml of sterile physiological saline. The cotton swab sampled by the soaking disinfection was placed in 10 ml of sterile physiological saline containing the corresponding neutralizing agent. The sample was shot 80 times in saline. Take 1ml of the washing solution for agar pouring and inoculation; set the temperature in a 37 Â°C incubator for 24 hours. Inoculate 2 plates per sample; calculate the number of uniform colonies. HBsAg pollution query; use ELISA Method detection.
The results of the experiment <br> The results showed that the sterilizer elimination rate was 99.88%; the 2% glutaraldehyde sterilization rate was 99.90%; the new germination sterilization rate was 97.65%; 75% alcohol sterilization rate was 98%. 88%.

Disinfection method	Number of inspections	Number of uniform colonies before disinfection (cfu/piece)	Number of uniform colonies before disinfection (cfu/piece)	Sterilization rate (Ã—10-2)
Sterilizer	18	1760	2	99.88
2% neutral glutaraldehyde	20	1876	2	99.90
0. 2% new clean	20	2160	51	97.65
75% alcohol scrub	20	2016	twenty three	98.88

Damage of HBsAg on the bearing; a total of 78 samples were tested; 10 HBsAg positives were detected before disinfection; the positive rate was 12.82%; after disinfection treatment; 3 positive samples were still detected after treatment with 0.2% neostigmine 2 positive samples were detected in 75% alcohol treated samples.

Disinfection method	Number of samples tested	Number of HBsAg positive samples (before disinfection)	Number of HBsAg positive samples (after disinfection)
Sterilizer	18	2	0
2% neutral glutaraldehyde	20	2	0
0. 2% new clean	20	3	3
75% alcohol scrub	20	3	2

Conclusion <br> Because the bearing layout is not suitable for high pressure steam sterilization. It can not be inactivated by 75% alcohol cotton ball scrubbing or 0. 2% Xinjieering soaking. In order to facilitate the damage without bearing damage This method is not advisable. Although it works well with 2% glutaraldehyde; it needs to be soaked for a long time; and it is irritating; it has damage to the instrument for a long time. The microwave sterilizer has better disinfection effect; Easy to use, the bearing disinfection operation can be completed between the two treatments; the HBsAg can be damaged; the corrosion is low; the bearing function does not change significantly after disinfection.
references
1. Chia WK, Dimensiomal change of impressions sterilization [J], Aust Dent J, 1990, 35 (1);
2. Ding Lanying; Jiang Li WBY-1 Microwave Sterilizer Research [J]; Chinese Journal of Disinfection; 1995; 12 (2), 65.

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