Abstract: The compatibility of different monomeric enzymes has different effects on the digestibility of non-starch polysaccharides in broiler wheat. In this experiment, in vitro simulated digestion method was used to study xylanase, β-glucanase, cellulase, phytase and mannanase, and the monomeric preparations of five monomeric enzyme preparations were added sequentially or in combination. The effect of adding dry matter and non-starch polysaccharide (NSP) digestibility on wheat based diets. The results showed that the five monomeric enzyme preparations had a synergistic effect on improving the dry matter and NSP digestibility, and the ratio of different compatibility was linear with the dry matter or NSP digestibility of the diet. The regression equations are as follows:
Dry matter digestibility:
Y1=-2.75e-2+18.5e2x1+46.5x2+11.5e2x3+47.8e2x4+2.7e2x5(R2=0.687*);
Soluble NSP (SNSP) Digestibility:
Y2=-3.18e3+19.4e2x1+4.4e2x2+13.6e2x3-2.96x4+6.92e2x5(R2=0.788*);
Insoluble NSP (INSP) digestibility:
Y3=2.5+7.18e3x1+14.18e2x2+11.18e2x3+4.5e2x4+58.2x5 (R2=0.763*).
The best combination is: xylanase 500 U·kg-1, β-glucanase 95 U·kg-1, cellulase 33 U·kg-1, phytase 540 U·kg-1, nectar The glycanase was 115 U·kg-1.
Key words: monomeric enzyme broiler in vitro digestion non-starch polysaccharide digestibility
With the improvement of people's living standards, the livestock and poultry breeding industry has developed rapidly. As of 2005, the total output of China's compound feed has reached 103 million tons. However, the continuous increase in feed production has brought about an increasingly tight supply and demand of feed ingredients. The price of feed ingredients continues to run at a high level. When the price of corn for feed raw materials is high, it is of practical significance to consider the use of wheat and other grains instead of corn.
However, non-starch polysaccharides (NSP) are commonly found in cereals such as wheat, barley and oats, which are the main anti-nutritional factors affecting the digestion and absorption of livestock, especially monogastric animals. Non-starch polysaccharide is a general term for all carbohydrates except starch in plant tissues, including cellulose, hemicellulose, pectin and resistant starch. NSP is usually divided into soluble non-starch polysaccharides (SNSP) and insoluble non-starch polysaccharides (INSP). Most of the literature believes that SNSP is the anti-nutritional effect, while INSP mainly plays a dilution role.
The anti-nutritional effects of NSP are summarized as follows:
1. The NSP of the plant feed cell wall is not digested by the animal itself, so that the intracellular starch, protein and other nutrients are difficult to digest and utilize, reducing the digestion and absorption of the feed nutrients, affecting the conversion efficiency of the diet and the animal's production performance;
2, causing changes in the morphology and physiology of the digestive tract of the animal, some water-soluble NSP can make the animal digestive organs increase or become heavier;
3, combined with certain physiologically active substances in the digestive tract, such as combined with digestive enzymes, bile salts, and even lipids, cholesterol, etc., thereby affecting the metabolism of lipids in the small intestine;
4, interaction with the microflora in the back of the digestive tract, causing anaerobic fermentation, producing a large amount of toxins, inhibiting animal growth;
5, the production of sticky manure, affecting the barn and the surrounding environment, laying chickens will also contaminate eggs.
The addition of NSP enzyme can degrade NSP into smaller aggregates and reduce its anti-nutrition. The NSP enzyme mainly includes xylanase, β-glucanase, cellulase, hemicellulase, pectinase, mannanase and the like. The surface of the wheat sample after enzyme treatment increased, the pore size increased, the intercellular layer was broken, and the digestive enzymes were easily introduced, thereby increasing the digestibility of dietary nutrients. The mechanism of action of the enzyme preparation is:
1, supplement the lack of endogenous enzymes, improve nutrient digestibility;
2, destroy plant cell walls, eliminate anti-nutritional factors;
3, hydrolyze non-starch polysaccharides, reduce intestinal chyme viscosity;
4, change the structure of the intestinal wall, improve the ability of nutrient absorption;
5. Improve the body's metabolic level and enhance immunity.
The addition of complex enzymes to the feed can increase the bird's weight by 7% to 10% and improve the feed conversion rate by 4% to 7%. When xylanase and β-glucanase are used in combination, the effect is superior to that of the single enzyme preparation, and it is assumed that further addition of monomeric enzyme preparations such as cellulase, mannanase and phytase is expected to be obtained. Good results.
In vitro simulation is one of the effective methods for evaluating the quality of enzyme preparations. ZyΙa et al. proposed a method for simulating the digestion of poultry, pouch, stomach and small intestine in vitro. Zhang Tieying and others believe that the application of phytase in vitro is feasible. In this experiment, the in vitro simulation method proposed by ZyΙa et al. was carried out to study the effects of adding five different enzyme preparations at different levels and different proportions on the dry matter and NSP digestibility of the diet in the broiler wheat basal diet, providing theory for the actual production of poultry. basis.
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